The purpose of this experimental investigation was to determine the effect to pulp response and healing process of amputation after topically treated by sodium fluoride and stannous fluoride.
The experiment was made on 36 molar from 12 experimental clogs, the average body weight about 16 Kg.
Experimental material was divided into two groups. Cavity formed group (Group I) and vital amputation group (Group II). Also, each group were subdivided into three sub-groups, according to the concentration of medicaments, 2%, 4% and 800.
The cavity were prepared class V on the buccal surfaces of the teeth and paid attention to keep cavity depth within 1 mm, from the´dentinoenamel junction. To relieve pain and normalize pulp response from cavity preparation, freshly cut cavity was sealed temporaly by Zinc-Oxide Eugenol cement for one week. After removal of temporary filling class V cavity and cut pulp surface were covered by a disk which saturated by 2,4 and 8% of sodium fluoride and stannous fluoride solution. Thereafter the cavity was sealed by Zinc-Oxide Eugenol.
The specimens were fixed in 10% formalin solution, decalcified with 5% nitric acid solution and processed for histopathologic examination.
The results were summarized as follows:
1. In the application of sodium fluoride of Group I, The odontoblastic layer of pulp tissue beneath the cavity was appeared formic degeneration(in 2;o application after 2 weeks), the inflammatory reaction with infiltration of leucocyte (4%, 4 weeks), and reticular atrophy (8%, 2 weeks).
2. In the application of stannous fluoride of Group I1, inain reaction were the loss of organized arrangement of odontoblastic layer (2%, 2 weeks) vacuolization of pulp tissue (4%, 4 weeks), and formation of heavily reticular atrophy. (8%, 2 weeks).
3. Group I. showed the formation of new dentin matrix only on the wall of root canal after 4 weeks.
4. In the application of sodium fluoride of Group Il, healing originating form the remaining pulp tissue. The typical form of this healing process is the closure of the root canal with a newly formed dentin bridge irregular (2%, 4 weeks).
5. In the application of stannous fluoride of Group II, the healing originating from the remaining pulp tissue. The main reaction were the closure of the root canal with irregularly formed dentin, (4%, 4 weeks), dentin bridge and pulpal reticular formation (4%, 8 weeks).
6. In the direct application of the surface of vital amputated pulp, the formation of dentin bridge was appeared after 4 weeks
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